HSA | FSA CARDS ARE NOW ACCEPTED

Last reviewed: May 12, 2026 Last updated: May 12, 2026

Written by: Jay Hastings , CEO of PlexusDx

Jay Hastings is the CEO of PlexusDx, a precision health company focused on genetic testing, blood biomarker insights, and personalized wellness recommendations. He has more than 20 years of experience across healthcare innovation, genomics, laboratory operations, healthcare investing, and strategic finance. His work has included scaling healthcare startups, leading CLIA lab integrations, and helping expand consumer access to precision health tools.

Medically reviewed by: Jayden Lee, PharmD, EMBA

Jayden Lee, PharmD, EMBA, is the PlexusDx Medical Science Liaison with a PharmD and MBA specializing in pharmacogenomics and clinical product development, with a proven ability to bridge the gap between genomic research and practical patient outcomes. Dr. Lee has more than 10 years of professional experience in clinical pharmacy, academia, and research.

Understanding Your DNMT3B Result: What It Means for Methylation and Health

The DNMT3B gene makes an enzyme called DNA methyltransferase 3B. This enzyme helps place methyl groups onto DNA, a process called de novo DNA methylation. Methylation controls gene activity, supports normal development, and helps maintain genomic stability. To add methyl groups, the enzyme uses SAM-e, converting it into homocysteine. Differences in DNMT3B activity can change how methyl groups are distributed in the body and can influence the Methionine Cycle and related methylation pathways.

Why This Matters

DNA methylation is a basic cellular process that supports many aspects of health including gene regulation, detoxification, nervous system function, and cell repair. If DNMT3B activity is higher or lower than typical, your body may handle nutrients involved in methylation differently. That can affect levels of SAM-e, homocysteine, folate, vitamin B12, and other cofactors that keep the Methionine Cycle balanced.

How to Read This Report

This report describes common genotypes for the DNMT3B variant rs2424913 and explains potential effects on methylation. The information is educational only. PlexusDx does not provide medical advice. Always consult your healthcare provider before making changes to diet, supplements, medications, or lifestyle based on genetic results.

Two effect alleles (TT) — Possible increased DNMT3B activity

If you have the TT genotype for rs2424913, you carry two copies of the effect allele. This variation may increase activity of the DNMT3B enzyme. Increased activity can change how your body uses methyl donors and may shift resource needs in the Methionine Cycle and Methylation Pathway.

Potential implications

  • Higher demand for SAM-e to supply methyl groups.
  • Possible increases in homocysteine if remethylation is insufficient.
  • Greater need for methylation cofactors such as folate and vitamin B12 to maintain balance.

Diet and food approach

  • Eat folate-rich foods: leafy greens, asparagus, Brussels sprouts, and legumes.
  • Include sources of vitamin B12: fish, shellfish, dairy, and eggs. Consider fortified foods if you eat a plant-based diet.
  • Consume foods that support SAM-e production: high quality protein sources like poultry, fish, and legumes provide methionine.
  • Limit excessive alcohol, as it can impair methylation and deplete B vitamins.

Supplements to consider discussing with your clinician

  • Methylfolate (5-MTHF) or folic acid if recommended by your provider.
  • Methylcobalamin (active B12) especially for low B12 or signs of deficiency.
  • B-complex with adequate B6, B12, and folate to support homocysteine clearance.
  • SAM-e under medical supervision if indicated for mood, liver, or joint support.

Lifestyle and monitoring

  • Regularly assess diet quality to ensure adequate B vitamins and protein.
  • Ask your clinician about testing homocysteine, serum folate, and B12 levels.
  • Manage stress and sleep, as both influence methylation pathways.
  • Stay active—exercise supports circulation and metabolic health which complements methylation.
One effect allele (CT) — Possible moderate increase in DNMT3B activity

If you have the CT genotype, you carry one copy of the effect allele. This may cause a milder, partial increase in DNMT3B activity. The effect is typically less pronounced than with two effect alleles but can still influence methylation and nutrient needs.

Potential implications

  • Moderate change in methylation demand and nutrient use.
  • Slightly higher need for methylation cofactors to maintain homocysteine balance.
  • Individual response varies depending on diet, other gene variants, and lifestyle.

Diet and food approach

  • Focus on a balanced diet with regular servings of leafy greens and vegetables high in folate.
  • Ensure adequate dietary B12, particularly if you follow a vegetarian or vegan diet.
  • Include quality protein sources for methionine while keeping overall intake balanced.
  • Prioritize whole foods and reduce processed food intake to support overall nutrient status.

Supplements to consider discussing with your clinician

  • Consider a B-complex if dietary intake is inconsistent.
  • Methylfolate or folic acid if tests show low folate or if recommended by a practitioner.
  • Methylcobalamin if B12 status is low or absorption is a concern.

Lifestyle and monitoring

  • Periodic blood testing for homocysteine, B12, and folate can help tailor recommendations.
  • Good sleep and stress reduction practices support methylation efficiency.
  • Regular physical activity and maintaining a healthy weight are beneficial.
No effect alleles (CC) — Typical DNMT3B activity

If you have the CC genotype, you carry two copies of the non-effect allele. This pattern is generally associated with typical DNMT3B activity and normal processing of nutrients involved in the Methionine Cycle. Your risk of methylation imbalance related to this variant is lower.

Potential implications

  • Standard requirements for SAM-e and methylation cofactors unless other factors apply.
  • Lower likelihood that this DNMT3B variant alone will create methylation imbalances.

Diet and food approach

  • Maintain a nutrient-rich diet that includes folate, B12, B6, and protein sources to support normal methylation.
  • Include a variety of vegetables, fruits, whole grains, legumes, and lean proteins.

Supplements to consider discussing with your clinician

  • Most people with typical activity rarely need targeted supplements specifically for this variant.
  • Consider standard multivitamin or B-complex if dietary intake is limited or if recommended by your clinician.

Lifestyle and monitoring

  • Routine preventive care including periodic nutrient screening as indicated by your provider.
  • Healthy habits such as regular exercise, stress management, and adequate sleep support methylation and overall health.

Other Factors That Affect Methylation

  • Diet quality and nutrient intake of folate, B12, B6, choline, and methionine.
  • Lifestyle factors such as alcohol use, smoking, sleep, stress, and physical activity.
  • Other genetic differences in methylation and one-carbon metabolism genes can change your risk profile.
  • Medications and health conditions that alter nutrient absorption or metabolism.

Practical Next Steps

  • Share these results with your healthcare provider and discuss whether testing for homocysteine, serum folate, and B12 is appropriate.
  • Review your diet with a clinician or registered dietitian to confirm you are getting adequate methylation nutrients.
  • Consider gentle lifestyle changes that support methylation: balanced meals, sleep, stress reduction, and regular activity.
  • If considering supplements, work with your provider to choose forms and doses tailored to your needs and safety.

PlexusDx provides genetic information to help you better understand potential predispositions. This information is educational only. PlexusDx does not provide medical advice. Always consult your healthcare provider before making changes to diet, supplements, lifestyle, or medical care based on genetic results.

If this genetic variant is present in your PlexusDx results, the following tests and reports are commonly used to explore it further:

🧬 Genetic Tests:

🧪 Blood Tests:

📄 Genetic Report:

Frequently Asked Questions About Methionine Cycle and DNMT3B rs2424913

What does the DNMT3B rs2424913 variant mean for methylation in my body?

DNMT3B helps carry out de novo DNA methylation, a process that controls gene activity and supports genomic stability. Your rs2424913 genotype may influence DNMT3B enzyme activity, which can alter how methyl groups are distributed and affect related one-carbon metabolism pathways like the Methionine Cycle. Depending on whether you have TT, CT, or CC, your body may use methylation nutrients (such as SAM-e, folate, and vitamin B12) differently to maintain balance.

How can rs2424913 TT, CT, or CC genotypes affect my SAM-e, homocysteine, folate, and B12 needs?

If you have TT, DNMT3B activity may be higher, potentially increasing demand for methyl donors (SAM-e) and methylation cofactors to support remethylation and homocysteine balance. If you have CT, the change may be moderate and nutrient needs may shift somewhat. If you have CC, DNMT3B activity is typically considered typical, so the likelihood of methylation imbalance driven by this variant alone is lower.

What diet, supplements, and tests are commonly recommended for DNMT3B-related methylation differences?

Diet approaches often focus on folate-rich foods (leafy greens, asparagus, Brussels sprouts, legumes), vitamin B12 sources (fish, shellfish, dairy, eggs; or fortified foods if plant-based), and quality protein for methionine. Because alcohol and lifestyle can affect methylation, limiting excessive alcohol and supporting sleep, stress, and activity are commonly emphasized. If appropriate, clinicians may consider blood testing such as homocysteine, serum folate, and vitamin B12, and may discuss supplements like methylfolate (5-MTHF), methylcobalamin (active B12), or a B-complex—always tailored to your results and safety with your healthcare provider.

What tests can help me learn more about Methionine Cycle and DNMT3B rs2424913?

The Genetic Methylation Test delivers over 300 genetic insights related to methylation, detoxification, and nutrient processing. The Methylation Pathway Genetic Report translates your results into personalized, actionable guidance. Your healthcare provider can also recommend targeted blood tests based on your specific pathway results and health history to complement your genetic insights with current biomarker data.

Medical and Editorial Standards

Medical review process: This article was reviewed for medical accuracy, scientific clarity, evidence alignment, and appropriate discussion of genetics, medications, supplements, biomarkers, and health-related claims.

Sources and evidence: PlexusDx educational content is developed using peer-reviewed research, clinical literature, reputable medical references, and, where applicable, public health or regulatory guidance. References are included at the end of the article when scientific, medical, or health-related claims are discussed.

Commercial transparency: PlexusDx offers genetic testing, blood biomarker testing, personalized supplement recommendations, and related precision wellness services. Product mentions are intended to help readers understand available options and should not be interpreted as medical advice.

Important disclaimer: PlexusDx educational content is for informational purposes only and should not be used as a substitute for professional medical advice, diagnosis, or treatment. Always consult a qualified healthcare provider before making decisions about medications, supplements, genetic testing, lab testing, or health-related care.